Asian Journal of Research and Reports in Hepatology

Aim: This study aimed at evaluating the antioxidant activities of Zinc, Selenium, and Vitamin B₁₂ on liver toxicity induced by Isoniazid-Rifampicin (INH-RIF).

Study Design: Original Research.

Place and Duration of Study: National Institute for Pharmaceutical Research and Development (NIPRD), Department of Pharmacology and Toxicology Idu, Abuja, Nigeria. Between August-September 2023.

Methods: 25 Swiss mice (25-35g) were divided into 5 groups, GP1 received distill water, GP 2-5 INH-RIF 150 mg/kg for the first two days; while groups 3 received Selenium 1 mg/kg, group 4 received VitaminB₁₂ 0.01 mg/kg, group 5 received Zinc 15 mg/kg respectively from day 3 till day 14 orally. Sera samples were obtained, and the organ (liver) was for weighing and histopathology on day 15.

Results: This study indicated that Selenium (1 mg/kg) showed reduction in the biochemical parameter ALT only; histo-anatomy of organ showed inflammation. Vitamin B₁₂ indicated reduction in ALT and ALP and no lesion seen in the organ, Zinc showed significant decrease in the level of ALP and ALT, and the histo-picture showed no lesion compared to INH-RIF group. Weights of the liver showed significant reduction in Selenium and Vitamin B₁₂ respectively compared to the toxic group.

Conclusion: It was concluded that Selenium 1 mg/kg, Vitamin B₁₂ and Zinc can mitigate toxic effects of Isoniazid-Rifampicin on the liver but Zinc indicated more ameliorative potentials. It was recommended that Zinc and /or Vitamin B₁₂ can be co-administered with these anti-tuberculars to mitigated the toxicity that may emanate from their administration.